Cyto-id® green detection reagent
WebDec 1, 2012 · The Cyto-ID green reagent (Cyto-ID) is a. ... Lyso-ID Red Detection Reagent (Lyso-ID) were from Enzo. Life Sciences. Cell culture. Bovine aortic endothelial cells (BAEC) were obtained from. WebApr 9, 2024 · CYTO-ID ® Green Detection Reagent 2 was stained for 30 min and analyzed by flow cytometry. Compared with untreated cells, the fluorescence was increased, with treatment with RAP + CLQ (Figures 4J,K). The results indicated that autophagy can be induced under stress conditions.
Cyto-id® green detection reagent
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WebThe CYTO-ID ® Red Tracer Dye fluorescence is independent of pH within normally encountered physiologic ranges and fluorescence intensity per cell is typically unaffected … WebIncase of Low CYTO-ID Green dye staining in all treatments, increase the reagent concentration (500X dilution of the dye is recommended) and the incubation time. ... grow the cells on coverslips after respective treatment incubate them with the dual detection reagent (Cyto‐ID dye and Hoechst 3342 nuclear stain) at 37 ºC for 30 minutes in the ...
WebDec 1, 2024 · The Cyto-ID® Green detection reagent which is a vital coloration of autophagic vacuoles can be read with a FITC filter (Excitation ∼480 nm, Emission ∼530), and the Hoechst 33,342 Nuclear Stain can be read with a DAPI filter set (Excitation ∼340, Emission ∼480). This result was examined using iRiS™ Digital Cell Imaging System … WebCyto-ID Green autophagy dye staining procedure for autophagy detection Cyto-ID Green solution was prepared by mixing the dye with 1× assay buffer. The cell sample was centrifuged, and the dye was pipetted in for image-based or flow cytometry, respectively. The sample was shielded from direct light and incubated for 30 min at 37°C,
WebJun 13, 2024 · Inflorescence-based of CYTO-ID® autophagy detection. Primary chondrocytes isolated from the cartilage of WT and GRN −/− mice were treated with DMSO (control) or IL-1β (1 μM) for 24 h, then stained with CYTO-ID® Green Detection Reagent and LysoTracker Red at a concentration of 300 nM was added and incubated at 37°C for … WebOct 1, 2024 · The autophagy level of haemocytes was detected with CYTO-ID® Autophagy Detection Kit (Enzo, Switzerland) according to the instruction. ... for 3 h, and then washed two times with M-L15 medium. The haemocytes on the slide were incubated with 200 μL CYTO-ID® Green Detection Reagent (diluted 1:500 in M-L15 medium) at 37 °C in dark …
WebCYTO-ID Autophagy detection kit (1x200tests) A no-transfection, quantitative assay for monitoring autophagy in live cells. CYTO-ID.The probe is a cationic amphiphilic tracer …
WebCyto-ID labeling and Flow cytometry: HEK-293 cells were harvested in Accutase solution (Merck Millipore, SF006), rinsed in DPBS and incubated for 30 min at room temperature … crystal change rodWebEnzo Life Sciences CYTO-ID Autophagy detection kit (1x50tests) A no-transfection, quantitative assay for monitoring autophagy in live cells. CYTO-ID.The probe is a cationic amphiphilic tracer (CAT) dye that rapidly … crystal channelingWebAug 6, 2024 · Autophagy analysis was performed by incubating cells with bafilomycin A1 for 30 min at 37 °C prior to treatment with CYTO-ID Green Detection Reagent, and then analyzing fluorescence by flow cytometry using the … dvs shoe company mens bootsWebThe CYTO-ID® Green Detection Reagent and the Autophagy Inducer (Rapamycin) contain DMSO which is readily absorbed through the skin. DMSO is harmful if ingested or … crystal channelWebFluorescent detection, HTS. Contents: CYTO-ID Green Detection Reagent. Hoechst 33342 Nuclear Stain. Autophagy Inducer (Rapamycin). Chloroquine Control. 10X Assay Buffer Catalog No. 50-103-1813 $558.00 / Each Qty Check Availability Add to cart Provide Content Correction dvs shoe company men\\u0027s sneakersWebThe LYSO-ID ® Green detection kit contains an acidic organelle-selective dye suitable for live-cell staining. Conventional fluorescent stains for acidic organelles, such as Acridine Orange (Prod. No. ENZ-52405), form … crystal chantellWebIn brief, cells were washed with PCR PBS (supplemented with 5% FBS) and then Gene Primer sequence mixed with Cyto-ID Dual Detection Reagent solution (Cyto-ID Green Detection Reagent and GAPDH Hoechst 33342). dvs shoe company mens flip flops