Psicheck vector
WebThe psiCHECK-2 Vector is a dual Luciferase vector designed to provide a quantitative and rapid approach for initial optimization of RNA interference (RNAi). The vector enable monitoring of changes in expression of a target gene fused to a reporter gene. The Renilla luciferase is used as the primary reporter gene, and the gene of interest is ... WebThe two recombinant vector plasmids were co-transfected with miR-181b mimics or miR-181b negative control (Negative Control, NC) using transfection reagent lipofectamineTM 2000, respectively. ... 组别 50nM 100 ng 1-3 hsa-miR-181b psiCHECK-TNF 4-6 Mir-NC psiCHECK-TNF 7-9 hsa-miR-181b psiCHECK-TNF-mut 10-12 Mir-NC psiCHECK-TNF-mut …
Psicheck vector
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WebPsicheck Tm 2 Vector, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Average 86 stars, based on 1 article reviews. Price from $9.99 to $1999.99. psicheck tm 2 vector - by Bioz Stars , 2024-04.
WebVector backbone psiCHECK-2 (Search Vector Database) Backbone manufacturer Promega Backbone size w/o insert (bp) 6273 Vector type Luciferase Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number High Copy Gene/Insert Gene/Insert name 3 miR-122 target sites Insert Size … WebThe psiCHECK™-2 Vector is designed to provide quantitative and rapid optimization of RNA interference (RNAi). The vector enables monitoring of changes in expression of a target …
WebFeb 15, 2024 · They were cloned into pSicheck-2.0 vector. We also cloned the common mode of the 3’-UTR region of BAP31 into pSicheck-2.0 (Fig. 3c ). These plasmids were co-transfected into 293T cells with... WebpsiCHECK-1 and psiCHECK-2 Vectors enable monitoring of changes in expression of a target gene fused to a reporter gene. They are used for optimizing RNA interference …
WebJul 7, 2024 · In this study, we replaced the SV40 promoter in the psiCHECK-2 reporter vector, which is widely used with mammalian cell lines, with the HSV-TK or AC5.1 promoter to yield two new dual-luciferase reporter vectors, designated psiCHECK-2-TK and psiCHECK-2-AC5.1, respectively. ... Moreover, psiCHECK-2-TK successfully detected the interaction ...
WebJul 7, 2024 · In this study, we replaced the SV40 promoter in the psiCHECK-2 reporter vector, which is widely used with mammalian cell lines, with the HSV-TK or AC5.1 promoter to … foxwoods slot machine wins youtubeWebDec 18, 2024 · psiCHECK-2-wt-EIF4EBP1 or psiCHECK-2-mut-EIF4EBP1. Two days later, luciferase activities were measured by Lucifer Reporter Assay System (Promega) and normalized to Renilla luciferase activity. Hematoxylin and eosin staining and immunohistochemical analysis foxwoods sign in to view accountWebpsiCHEC-2 Vector is designed to provide a quantitative and rapid approach for optimization of RNA interference (RNAi). The vectors enable the monitoring of changes in expression of a target gene fused to the reporter gene. foxwoods slots youtubeWebAug 4, 2024 · We chose wild-type (WT) STC2-3′UTR or mutant (MUT)-STC2-3′UTR to set up a two-tier luciferase assay with psiCHECK-2 vector. ... The level of miRNA-8516 was recovered after co-transfection pCD5-circRNA8220 vector and miRNA-8516. miR-8516 increased after silencing the expression of circRNA8220 using small interfering RNAs ... foxwoods smoke freeWebThe psiCHECK™-1 Vector(a–d) (Cat.# C8011) and psiCHECK™-2 Vector(a–f) (Cat.# C8021) are designed to provide a quantitative and rapid approach for optimization of RNA … blackwood rotary art show 2022 salesWebShuffle T7 Express E.coli Strain大肠杆菌表达菌株及感受态细胞. 价 格: ¥0. 货 号: 3544763. 产 地: 北京. 点击询问 我要采购. 竭诚为您服务!. foxwoods site maphttp://www.honorgene.com/uploads/files/202404/5ea518d17dd8f.pdf foxwoods slot tournament