WebObtain fast, simple, and highly reproducible trypsin protein digestion for peptide characterization and quantitation in biopharmaceutical protein research. Shop Thermo … WebSMART Digest kit protocol 50 µL rituximab sample, adjusted to 2 mg/mL with water, was diluted 1:4 (v/v) with the SMART Digest buffer provided with the kit. It was then transferred to a reaction tube containing 15 µL of the SMART digest resin slurry, corresponding to 14 µg of heat-stabile, immobilized trypsin.

SMART Digest™ Immunoaffinity (IA) Protein G Kits

WebApr 11, 2024 · Materials & methods: The performance of commercially available next-generation trypsins was assessed based on the digestion of protein standards in buffer and complex matrix by LC–high-resolution MS. Results: The performance of all next-generation trypsins assessed exceeded that of an overnight tryptic digest in a fraction of the time. WebTo demonstrate how Thermo Scientific™ SMART Digest™ kits remove uncertainty associated with conventional solution-based tryptic digestion protocols, resulting in higher reproducibility and sample characterization. Trypsin digestion of insulin using the SMART Digest kit and a series of commonly used buffer species and additives were compared. highlands pharmacy fayetteville ar

SMART Digest Kit FAQs - Fisher Sci

WebThermo Fisher Scientific WebThe SMART Digest kit provides significant improvements in reproducibility over existing protocols, which results in fewer sample failures, higher throughput and the ability to more easily interrogate data. The following chromatogram shows 13 overlaid traces of mouse mAb peptides, digested with the SMART Digest kit. small marginal endplate osteophytes